INDUCTION OF MICROTUBING OF NEW PROMISING POTATO VARIETIES IN ASEPTIC CULTURE

نویسندگان

چکیده

The studies were carried out with the aim of optimizing microtubing promising potato varieties in aseptic culture. experiments studied such factors as use vessels various sizes (test tubes a diameter 25 mm and plastic containers 18x18 cm, into which 10 ml agar 400 liquid nutrient medium, respectively, poured, respectively), density planting plants (40, 60 80 stem explants), composition medium during ontogenesis, cultivation mini-tubers from vitro microplants (control) microtubers (? 0.9 cm 0.5 ... size). When studying possibility modifying based on Murashige-Skoog recipe to induce tuberization one variant, sucrose concentration growth was changed 2% before formation four internodes by 8% after this phase, kinetin content entire observation period mg/l. In second kept 6% 0.25 mg/l throughout ontogenesis. highest yield standard fraction (27 94%) multiplication factor 0.8 2.7 pcs/plant noted variant cuttings placed container. grown test change 1.0 1.5 collected per plant 64 78%. container technology similar alternation media increased most 75 86%. variants constant (6%), very low noted, did not compensate for sufficiently high fraction, regardless laboratory vessel used. tube ground higher than when microtubers,

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ژورنال

عنوان ژورنال: Vestnik of Kazan State Agrarian University

سال: 2021

ISSN: ['2073-0462']

DOI: https://doi.org/10.12737/2073-0462-2021-48-54